Degree Name
MS (Master of Science)
Program
Biology
Date of Award
12-2018
Committee Chair or Co-Chairs
Dr. Yongke Lu
Committee Members
Dr. Allan Forsman, Dr. Jonathan Peterson, Dr. Jim Stewart
Abstract
Excessive alcohol consumption is the primary contributing factor in the development of alcoholic liver diseases (ALD). Nicotine contained in tobacco is a major addictive alkaloid, which enhances the effects of ALDs. The major enzyme involved in nicotine metabolism is cytochrome P450 2A5 (CYP2A5) which is produced in the liver. Alcohol can stimulate the CYP2A5 enzyme. We utilized cyp2a5-/- knockout mice in this research to examine the effects of CYP2A5.
The cyp2a5-/- mice and wild-type (WT) mice were fed liquid ethanol diet with or without nicotine to induce ALD. Nicotine enhancing effects on ALD were observed in WT mice but not in cyp2a5-/- mice. Oxidative stress was stimulated by alcohol and further increased by nicotine in WT mice but not in cyp2a5-/-mice. Microsomal ROS production during microsomal metabolism of nicotine was increased in WT mice but not in cyp2a5-/-mice. These results suggest that nicotine enhances ALD is CYP2A5 dependent.
Document Type
Thesis - unrestricted
Recommended Citation
Owoseni, Seun Emmanuel, "The Study of Alcoholic Liver Diseases" (2018). Electronic Theses and Dissertations. Paper 3493. https://dc.etsu.edu/etd/3493
Copyright
Copyright by the authors.
Included in
Anatomy Commons, Animal Sciences Commons, Biochemistry, Biophysics, and Structural Biology Commons, Biology Commons, Chemicals and Drugs Commons, Medical Sciences Commons