MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Dr. Greg Bishop
Dr. Dane W. Scott, Dr. Catherine McCusker
Biomarkers are measurable indicators of health status or disease state that can be used for diagnosis and may help guide patient treatment strategies. Enzyme-linked immunosorbent assays (ELISA) and other many clinical techniques currently used for measuring biomarker proteins lack sensitivity, demand high analysis cost, are often not well-suited for measuring multiple biomarkers in a single sample, and require long analysis times. Here, we demonstrate simple, low-cost 3D-printed flow-through devices with integrated electrodes modified with gold nanoparticles (AuNPs) for electrochemical immunoassays of S100B, a biomarker protein related to conditions like skin cancer and brain injuries. Flow-through devices are fabricated from photocurable-resin using a desktop digital light processing (DLP) projector-based 3D printer to produce 500-800 µm square cross-sectional fluidic channels. Threaded ports at the ends and center of the channel are included in the device design for connecting commercially available fittings for fluid delivery and integrating low-cost graphite electrodes for electrochemical biosensing.
Thesis - unrestricted
Alabdulwaheed, Abdulhameed, "3D-Printed Fluidic Devices and Incorporated Graphite Electrodes for Electrochemical Immunoassay of Biomarker Proteins" (2018). Electronic Theses and Dissertations. Paper 3477. https://dc.etsu.edu/etd/3477
Copyright by the authors.