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MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Cecilia McIntosh, Ranjan Chakraborty
Fatty acid amide hydrolase (FAAH) is an enzyme that terminates the signaling role played by the lipid mediators, N- acylethanolamines (NAEs), present both in plants and animals. FAAH is responsible for NAE hydrolysis and has been extensively studied in mammalian systems and the model plant Arabidopsis thaliana; it has been reported in various organisms as well as some crop plants such as rice and Medicago truncatula. To understand the role of FAAH in diverse organisms, here we report the identification and biochemical characterization of a FAAH homolog in tomato. Previously identified and cloned candidate FAAH from tomato was expressed in Escherichia coli as a fused protein with 6X his-tag for identification. Supernatant containing recombinant FAAH showed the ability to hydrolyze NAE substrates. The optimal reaction conditions for enzyme assay and kinetic parameters for tomato FAAH were determined and effect of inhibitor on enzyme was determined. Characterization of FAAH in tomato will contribute to further understanding of NAE metabolic pathway and its implications.
Thesis - Campus Only
Shrestha, Sujan, "Biochemical Characterization of Tomato Fatty Acid Amide Hydrolase" (2018). Electronic Theses and Dissertations. Paper 3469. https://dc.etsu.edu/etd/3469
Copyright by the authors.