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Degree Name

MS (Master of Science)

Program

Biology

Date of Award

8-2018

Committee Chair or Co-Chairs

Aruna Kilaru

Committee Members

Cecilia McIntosh, Ranjan Chakraborty

Abstract

Fatty acid amide hydrolase (FAAH) is an enzyme that terminates the signaling role played by the lipid mediators, N- acylethanolamines (NAEs), present both in plants and animals. FAAH is responsible for NAE hydrolysis and has been extensively studied in mammalian systems and the model plant Arabidopsis thaliana; it has been reported in various organisms as well as some crop plants such as rice and Medicago truncatula. To understand the role of FAAH in diverse organisms, here we report the identification and biochemical characterization of a FAAH homolog in tomato. Previously identified and cloned candidate FAAH from tomato was expressed in Escherichia coli as a fused protein with 6X his-tag for identification. Supernatant containing recombinant FAAH showed the ability to hydrolyze NAE substrates. The optimal reaction conditions for enzyme assay and kinetic parameters for tomato FAAH were determined and effect of inhibitor on enzyme was determined. Characterization of FAAH in tomato will contribute to further understanding of NAE metabolic pathway and its implications.

Document Type

Thesis - Campus Only

Copyright

Copyright by the authors.

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