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MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Tom W. Ecay, Cecilia McIntosh
Corneal endothelial cells have been shown to possess a uniform polygonal and complex multipolar shape at their apical and basolateral surface respectively. We established a morphological timetable to study how this complex shape arises postnatally. Corneas were collected from mice between postnatal day 8 to postnatal day 35 and labelled with antibodies specific for ZO-1 and NCAM at apical and basolateral region, respectively. Images were collected using wide-field fluorescence microscopy and morphometrically analyzed. Results showed that apical cell sizes increase linearly over the first 3 weeks, plateauing at 4-5 weeks postnatally with increased regularity. Basolateral membrane surfaces remained relatively smooth prior to eyelid opening and thereafter begins developing showing differences in development from periphery to the center until about 4 weeks postnatally when the wavy processes get vivid. Results indicate concurrent and independent development at both poles of the corneal endothelium, with more complexity seen at the basolateral surface.
Thesis - restricted
Ojo, Victor Temidayo, "Postnatal Cell Shape development of the Corneal Endothelium in Mice" (2017). Electronic Theses and Dissertations. Paper 3301. https://dc.etsu.edu/etd/3301
Copyright by the authors.