MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Aruna Kilaru, Leonard Robertson
Phylloquinone is a natural analog of vitamin K that has been shown to both inhibit cancer cell growth and induce apoptosis in several cancer cell lines. This study examined these effects in a non-Hodgkin lymphoma cell line, known as U937. Cell growth inhibition and apoptosis were assessed through the quantification of cell density and area, following treatment with several concentrations of phylloquinone. In addition, apoptosis was detected and quantified using immunofluorescent markers of apoptosis (i.e. annexin V, APO-BrdU). Treatment with phylloquinone resulted in reduced overall cell density, increased overall cell area, and an increased frequency of apoptosis in U937 cells. Increasing both phylloquinone concentration and treatment time enhanced these effects. These results are significant because they document the anti-cancer effects of this analog of vitamin K, as well as provide insight into the morphological changes that occur during apoptosis in U937 cells.
Thesis - Open Access
Blair, Tesha E., "The Apoptotic and Inhibitory Effects of Phylloquinone in the U937 Cell Line" (2016). Electronic Theses and Dissertations. Paper 3028. https://dc.etsu.edu/etd/3028
Copyright by the authors.