MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Dhirendra Kumar, Karl H. Joplin
A variety of plants synthesize and store oil in the form of triacylglycerols (TAG) in their seed and nonseed tissues that are commonly used as vegetable oils. In seed tissues, an acyl CoA-dependent diacylglycerol (DAG) acyltransferase (DGAT) and/or -independent phospholipid:DGAT (PDAT) catalyze the conversion of DAG to TAG. In avocado fruit, which stores up to 70% oil by dry weight in mesocarp, it is hypothesized that both DGAT and PDAT are likely involved in TAG synthesis. To investigate, TAG content and composition and transcript levels for the acyltransferases in avocado fruit were quantified by gas chromatography and real-time polymerase chain reaction, respectively. Temporal, tissue-specific and phenotypic comparisons revealed that while DGAT1 gene expression was specifically associated with TAG accumulation, PDAT also correlated with higher levels of polyunsaturated fatty acid; DGAT2 was barely detectable. These studies suggest that TAG biosynthesis in nonseed tissues of avocado involves acyl CoA-dependent and -independent reactions.
Thesis - Open Access
Sung, Ha-Jung, "Identification of Acyltransferases Associated with Triacylglycerol Biosynthesis in Avocado" (2013). Electronic Theses and Dissertations. Paper 2311. https://dc.etsu.edu/etd/2311
Copyright by the authors.