MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Ismail O. Kady, Scott J. Kirkby
A simple HPLC procedure for the quantitative determination of citral, the major fragrant component in the lemon grass, has been developed. The procedure involves a C-8 stationary phase using a 90:10 methanol: water pH 5 mobile phase containing 0.25% 1-octanesulfonic acid and an UV detector (set at 233 nm). The lemon grass leaves were harvested fresh at different times of the year and were soaked in methanol for 48 hours without any mechanical assistance to extract the citral and other methanol soluble components. The method showed good reproducibility with relative standard deviation of 2.8% and 10.8% for two different sets of samples. The method showed linearity in the range of 0.89 - 35.52 μg/mL. The average recovery was 104.8%. The amount of citral found as a percentage of the dried leaves are, 0.093, 0.27, 0.10, 0.13, 0.16, and 0.066 for fall, winter, three summer, and 1 commercial store samples, respectively.
Thesis - unrestricted
AL-Shaer, Mahmoud, "Reversed-Phase HPLC Determination of Citral in Locally Grown Lemon Grass Harvested at Different Season." (2006). Electronic Theses and Dissertations. Paper 2229. https://dc.etsu.edu/etd/2229
Copyright by the authors.