Off-campus ETSU users: To download "Campus Only" theses, please use the following link to log in to our proxy server with your ETSU username and password.
Non-ETSU users: Please talk to your librarian about requesting this thesis through interlibrary loan.
Degree Name
MS (Master of Science)
Program
Biology
Date of Award
5-2005
Committee Chair or Co-Chairs
Lee M. Pike, Cecilia A. McIntosh
Committee Members
Hugh A. Miller III
Abstract
Flavonoids are chemically modified by glucosylation, hydroxylation, methylation, etc. During glucosylation, the sugar moiety from UDP-sugar is transferred to aglycone flavonoid substrates by glucosyltransferases (GTs). Grapefruit contains 5 different glucosyltransferases that demonstrate differences in not only substrate but also position specificity. Previous research obtained 3 putative 5’ grapefruit GT clones using SMART RACE RT-PCR with a degenerate gene specific primer based on a highly conserved sequence area in the Plant Secondary Product Glucosyltransferase box. The objective of this research was to use clone specific primers to obtain 3’ clones of the 3 previously mentioned 5’ clones as well as verify putative GT candidacy based on sequence data. Two of the 3 putative GT candidates were designated non-GTs following 3’end sequencing. During pursuit of sequence for the remaining 5’ clone, 1 full-length clone and 1 partial putative GT clone were obtained. To verify GT status, the clones must undergo expression/biochemical characterization.
Document Type
Thesis - restricted
Recommended Citation
Strong, Christy, "Flavonoid Glucosyltranferases: Cloning and Sequencing of Putative Glucosyltranferases from Citrus paradisi (Grapefruit) Leaves." (2005). Electronic Theses and Dissertations. Paper 989. https://dc.etsu.edu/etd/989
Copyright
Copyright by the authors.