Degree Name
MS (Master of Science)
Program
Biology
Date of Award
8-2004
Committee Chair or Co-Chairs
Ranjan N. Chakraborty
Committee Members
Bert C. Lampson, Lee M. Pike
Abstract
Many gram-negative bacteria produce and secrete siderophores under iron-deficient conditions. Siderophores are low molecular weight compounds (600-1500 Daltons), which chelate ferric iron with an extremely high affinity, and the complex is actively transported across the outer and inner membranes of gram-negative bacteria. There are two main classes of siderophores: catechol and hydroxamate. Catechol-type siderophores chelate ferric iron via hydroxyl groups, and hydroxamate-type siderophores chelate ferric iron via a carbonyl group with an adjacent nitrogen. Rhizobia fix atmospheric nitrogen symbiotically in leguminous plants using the iron-containing enzyme nitrogenase. To satisfy their iron requirements, many rhizobia are known to produce siderophores. Rhizobium leguminosarum Strain IARI 312 is known to infect pigeon pea plants. R. leguminosarum Strain IARI 312 produces both a catechol-type and a hydroxamate-type siderophore when grown under iron deficient conditions. The catechol-type siderophore has been purified and chemically characterized, and is consistent with that of enterobactin.
Document Type
Thesis - unrestricted
Recommended Citation
Clark, Brianne Lee, "Characterization of a Catechol-Type Siderophore and the Detection of a Possible Outer Membrane Receptor Protein from Rhizobium leguminosarum strain IARI 312." (2004). Electronic Theses and Dissertations. Paper 922. https://dc.etsu.edu/etd/922
Copyright
Copyright by the authors.