Degree Name

MS (Master of Science)

Program

Biomedical Sciences

Date of Award

12-2003

Committee Chair or Co-Chairs

Phillip R. Musich

Committee Members

Yue Zou, Krishna Singh, David A. Johnson

Abstract

Dietary flavonoids are ubiquitous and are marketed as supplements. Characterized as antioxidants, they offer protection against a number of degenerative diseases. Flavonoid mechanics involve free radical scavenging, metal chelation, and substrate association. The skeletal structure of flavonoids is a fused ring system modified by hydroxyl, sugar, and carbohydrate additions. Flavone is a structurally simple flavonoid. Quercetin and its glycosidic analog rutin are complex structures. Using a DNA oxidation/cleavage assay, flavone reduces DNA nicking by 91%. Depending on the solvent system used, quercetin can either increase or decrease DNA oxidation. Rutin exhibits neither pro- nor antioxidant activity. The molecular interactions responsible for these results are defined for flavone. 1) Flavone intercalates into DNA and saturates DNA at a 1/3.5 flavone:DNA molar ratio. 3) Flavone reduces iron-dependent DNA oxidation. 4) Flavone interacts with quercetin to enhance DNA protection. These results characterize the primary activities of a simple flavonoid.

Document Type

Thesis - Open Access

Copyright

Copyright by the authors.

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