Degree Name
MS (Master of Science)
Program
Biology
Date of Award
8-2026
Committee Chair or Co-Chairs
Ranjan Chakraborty
Committee Members
Erik Petersen, Laraine Powers
Abstract
Iron is essential for many biological processes, where it is used as a cofactor. Even though it is abundant in the Earth’s crust, it is in an insoluble form unavailable to microorganisms in the soil. To overcome this, bacteria produce siderophores, which are secondary metabolites that have a high affinity for iron. This allows bacterial cells to obtain insoluble iron that is essential for survival. Pseudomonas mosselii, a Gram negative bacterium, was isolated from soil. It was selected for further analysis as it was found to produce siderophore(s) under iron-limited conditions, which have not been characterized previously. Therefore, we aimed to purify and structurally characterize the siderophore(s) produced by P. mosselii. Our research revealed a catechol-type siderophore with fluorescence, and preliminary structural analysis revealed the presence of two molecules: 2,3-dihydroxybenzoic acid and 2,3-dihydroxybenzoic acid + methyl serine.
Document Type
Thesis - unrestricted
Recommended Citation
Rasnake, Kemper R., "Characterization of Catechol-Type Siderophore(s) from P. mosselii CFML 90-83" (2026). Electronic Theses and Dissertations. Paper 4730. https://dc.etsu.edu/etd/4730
Copyright
Copyright by the authors.
