Degree Name
MS (Master of Science)
Program
Biology
Date of Award
5-2018
Committee Chair or Co-Chairs
Ranjan Chakraborty
Committee Members
Christopher Pritchett, Cecilia A. McIntosh
Abstract
Siderophores are low molecular weight, iron chelating compounds produced by many bacteria for uptake of iron in case of iron scarcity. Vicibactin is a trihydroxamate type siderophore produced by Rhizobium leguminosarum bv. trifolii ATCC 14479. This work focuses on identifying an outer membrane receptor involved in the transport of vicibactin. We have confirmed the presence of the putative fhuA gene in R. leguminosarum bv. trifolii ATCC 14479. This bacteria shows mutualistic symbiosis with the red clover plant Trifoliium prantense. Leghemoglobin, with its cofactor heme is present in the plant root nodules that surrounds the infecting organism present in the nodules. This work attempts to elucidate the ability of Rhizobium leguminosarum bv. trifolii ATCC 14479 to utilize heme-bound iron and genes involved in the transport. We have also elucidated the role of energy transducing proteins TonB- ExbB-ExbD on the heme-bound iron uptake system.
Document Type
Thesis - unrestricted
Recommended Citation
Khanal, Sushant, "Identification of “fhuA” Like Genes in Rhizobium leguminosarum ATCC 14479 and its Role in Vicibactin Transport and Investigation of Heme Bound Iron Uptake System" (2018). Electronic Theses and Dissertations. Paper 3354. https://dc.etsu.edu/etd/3354
Copyright
Copyright by the authors.