MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Cassandra T. Eagle
David Wiliams, Michael Kruppa, Marina Roginskaya
C. albicans is a polymorphic fungal pathogen which has the ability to shift from yeast to hyphae. C. albicans cell wall is composed of glucan, chitin, mannoprotein and mannan. It is not possible, using standard extraction methods, to isolate mannan from C. albicans hyphae. To isolate hyphal mannan, we developed a simplified alkali extraction method. Using this method it was determined that hyphal mannan has a much lower molecular weight, a smaller polymer distribution and altered conformation structure when compared to yeast mannan. The hyphal mannan was found to contain little to no acid-labile portion with only α-Man-PO4 groups and no long chains of β-1, 2-linked mannosyl repeat units, when compared to the yeast mannan. It was concluded that the C. albicans hyphal mannan is substantially different from the mannan found in the yeast form. This is an entirely new observation that extends the existing knowledge about the structural biology of C. albicans hyphae and may provide insights into the role of hyphae in pathogenesis.
Thesis - Open Access
Kwofie, Francis, "Candida albicans Hyphal Mannan is Structurally Distinct from Yeast Mannan" (2015). Electronic Theses and Dissertations. Paper 2555. http://dc.etsu.edu/etd/2555
Copyright by the authors.