Degree Name

MS (Master of Science)

Program

Biology

Date of Award

5-2015

Committee Chair or Co-Chairs

Aruna Kilaru

Committee Members

Lev Yampolsky, Cecilia Mcintosh

Abstract

This work identifies transcription factors (TF) controlling triacylglycerol (TAG) synthesis and accumulation in plant tissues. TAG plays vital role in plants and are used by humans. Most plants accumulate oil in the seed, but some species accumulate oil in other tissues. The Wrinkled1 (WRI1) TF has been shown to regulate oil accumulation in multiple species and tissues. Here, four WRI homologues in avocado were identified, their phylogeny was examined and three of them were cloned into expression vectors for further characterization. However, WRI1 likely does not act alone in regulation of TAG accumulation in plants. Additional candidate TFs were identified by using transcriptome data from a variety of species, and cloned into expression vectors. Future studies will be able to use this information to better understand regulation of TAG accumulation, which will allow increased oil accumulation in plants for various human uses.

Document Type

Thesis - Open Access

Copyright

Copyright by the authors.

Included in

Biology Commons

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