Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
5-2015
Committee Chair or Co-Chairs
Sharon Campbell
Committee Members
William Stone, Cassandra Eagle
Abstract
Vitamin E exhibits anti-tumor activity by regulating pathways in cancer cells, potentially the lipoxygenase (LOX) pathway. We studied the effects of alpha tocopherol (AT), gamma tocopherol (GT), gamma tocotrienol (GT3), and an alpha-gamma tocopherol mixture (ATGT) on the production of the LOX metabolites 13-hydroxyoctadecaenoic acid (HODE), 15-hydroxyeicosatetraenoic acid (HETE), 12-HETE, and 5-HETE in colorectal cancer. These metabolites were examined in the HCT-116 cell line after 24 h treatment with select vitamin E isoforms and quantified by LC/MS/MS. Under physiological conditions, we find that treatment with varying vitamin E isoforms have different effects on the production of 13-HODE, 15-HETE, 12-HETE, and 5-HETE. GT increases 13-HODE and decreases 12-HETE. AT reverses the effects of GT regulation on the LOX pathway, while GT3 has no significant effect on the metabolites tested. GT shows superiority in regulating the LOX pathway as it increases 13-HODE and decreases 12-HETE for possible prevention of colorectal cancer.
Document Type
Thesis - unrestricted
Recommended Citation
Borketey, Martha A., "Effects of Select Vitamin E Isoforms on the Production of Polyunsaturated Fatty Acid Metabolites in Colorectal Cancer" (2015). Electronic Theses and Dissertations. Paper 2480. https://dc.etsu.edu/etd/2480
Copyright
Copyright by the authors.