MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Cecilia A. McIntosh, Bert C. Lampson
Salicylic acid binding protein 2 (SABP2) that converts methyl salicylate to salicylic acid (SA) plays an obligatory role in the SA-mediated disease resistance pathway in plants. SABP2 interacts with SBIP24 in a yeast two-hybrid screening. SBIP24 belongs to the stearoyl-acyl carrier protein-desaturase protein family. To biochemically characterize the SBIP24, it was cloned from tobacco leaves using RT-PCR and expressed in E. coli. Recombinant SBIP24 was affinity purified using Ni-NTA chromatography. RT-PCR was performed to determine the role of SABP2 in modulating the expression SBIP24. TMV infected transgenic C3 (control tobacco plant containing empty silencing vector) and 1-2 (SABP2-silenced) transgenic tobacco plants were used. Preliminary results indicate that SABP2 may regulate the expression of SBIP24 in tobacco plants. Further studies are needed to confirm these preliminary results.
Thesis - Open Access
Ferdous, Amin J., "Cloning and Expression of a Tobacco Stearoyl-ACP Desaturase Gene SBIP24 and its Interaction with SABP2 in SA pathway" (2014). Electronic Theses and Dissertations. Paper 2429. http://dc.etsu.edu/etd/2429
Copyright by the authors.