Degree Name
MS (Master of Science)
Program
Biology
Date of Award
12-2014
Committee Chair or Co-Chairs
Dhirendra Kumar
Committee Members
Cecilia A. McIntosh, Bert C. Lampson
Abstract
Salicylic acid binding protein 2 (SABP2) that converts methyl salicylate to salicylic acid (SA) plays an obligatory role in the SA-mediated disease resistance pathway in plants. SABP2 interacts with SBIP24 in a yeast two-hybrid screening. SBIP24 belongs to the stearoyl-acyl carrier protein-desaturase protein family. To biochemically characterize the SBIP24, it was cloned from tobacco leaves using RT-PCR and expressed in E. coli. Recombinant SBIP24 was affinity purified using Ni-NTA chromatography. RT-PCR was performed to determine the role of SABP2 in modulating the expression SBIP24. TMV infected transgenic C3 (control tobacco plant containing empty silencing vector) and 1-2 (SABP2-silenced) transgenic tobacco plants were used. Preliminary results indicate that SABP2 may regulate the expression of SBIP24 in tobacco plants. Further studies are needed to confirm these preliminary results.
Document Type
Thesis - unrestricted
Recommended Citation
Ferdous, Amin J., "Cloning and Expression of a Tobacco Stearoyl-ACP Desaturase Gene SBIP24 and its Interaction with SABP2 in SA pathway" (2014). Electronic Theses and Dissertations. Paper 2429. https://dc.etsu.edu/etd/2429
Copyright
Copyright by the authors.