Degree Name

MS (Master of Science)

Program

Chemistry

Date of Award

8-2007

Committee Chair or Co-Chairs

William Stone

Committee Members

Hamid S. Kasmai, Chu Ngi Ho

Abstract

Proteomics is the large scale study of proteins in cells or organisms. The purpose of this study was to characterize the proteomic alterations occurring in a prostate cancer (LNCaP) cell line after treatment with delta-tocotrienol (a form of vitamin E not very prevalent from most dietary sources). We found that both gamma- and delta-tocotrienols induced time and concentration dependent growth inhibition and programmed cell dead (apoptosis) in LNCaP cells. Secondly, we used two-dimensional gel electrophoresis (2-DE) to characterize changes in protein expression levels associated with this treatment. Our results show that a specific set of proteins are regulated at both early and late times following treatment with delta-tocotrienol and these proteins have been characterized by their apparent molecular weights and isoelectric points. The alteration observed at early time points are particularly interesting because these changes are likely to reflect the underlying molecular mechanisms for triggering cancer cell death.

Document Type

Thesis - Open Access

Copyright

Copyright by the authors.

Included in

Cell Biology Commons

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