Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
8-2007
Committee Chair or Co-Chairs
William Stone
Committee Members
Hamid S. Kasmai, Chu Ngi Ho
Abstract
Proteomics is the large scale study of proteins in cells or organisms. The purpose of this study was to characterize the proteomic alterations occurring in a prostate cancer (LNCaP) cell line after treatment with delta-tocotrienol (a form of vitamin E not very prevalent from most dietary sources). We found that both gamma- and delta-tocotrienols induced time and concentration dependent growth inhibition and programmed cell dead (apoptosis) in LNCaP cells. Secondly, we used two-dimensional gel electrophoresis (2-DE) to characterize changes in protein expression levels associated with this treatment. Our results show that a specific set of proteins are regulated at both early and late times following treatment with delta-tocotrienol and these proteins have been characterized by their apparent molecular weights and isoelectric points. The alteration observed at early time points are particularly interesting because these changes are likely to reflect the underlying molecular mechanisms for triggering cancer cell death.
Document Type
Thesis - unrestricted
Recommended Citation
Muenyi, Christian Mbangha, "Vitamin E (Tocotrienols) and Prostate Cancer: A Proteomics Approach." (2007). Electronic Theses and Dissertations. Paper 2126. https://dc.etsu.edu/etd/2126
Copyright
Copyright by the authors.