Honors Program
Honors in Health Sciences: Microbiology
Date of Award
5-2020
Thesis Professor(s)
Dr. Dhirendra Kumar
Thesis Professor Department
Biological Sciences
Thesis Reader(s)
Dr. Sean Fox
Abstract
Subcellular Localization of Tobacco SABP2 under Normal and Stress Conditions
Salicylic acid (SA), a phytohormone, plays an important role in plant physiology. SA mediated innate immune pathway is an important pathway for plant immunity against pathogens. Plants resisting pathogen infection synthesize higher levels of Methyl Salicylate (MeSA), which is then converted to SA by the esterase activity of Salicylic Acid Binding Protein 2 (SABP2). The high level of the converted SA leads to enhanced pathogen resistance. The study of subcellular localization of a protein is critical in explaining its potential biochemical functions. SABP2 tagged with eGFP was expressed transiently in Nicotiana benthamiana leaves. The SABP2-eGFP expressing leaves were challenged with bacterial and viral pathogens and observed under confocal microscopy. Fluorescent signals were seen throughout the cell and more concentrated towards the cell periphery. To verify the localization, mCherry fluorescent organelle markers with specific targeting sequences were used. The results indicate that the SABP2 is likely a cytoplasmic protein, and there is no change in its localization upon infection by plant pathogens.
Publisher
East Tennessee State University
Document Type
Honors Thesis - Open Access
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Recommended Citation
Das, Sanjeev, "Subcellular Localization of Tobacco SABP2 under Normal and Stress Conditions" (2020). Undergraduate Honors Theses. Paper 569. https://dc.etsu.edu/honors/569
Copyright
Copyright by the authors.
Included in
Biochemistry Commons, Botany Commons, Molecular Biology Commons, Molecular Genetics Commons, Plant Biology Commons, Plant Pathology Commons, Plants Commons