Candida Albicans Gene Expression in an in Vivo Infection Model
A general procedure is described for the analysis of gene expression of Candida albicans cultured in a mouse infection model. This technique involves first infecting mice with Candida and subsequently harvesting blood and other tissue at specific time points during infection. The tissues are homogenized and the infecting Candida isolated. Finally, RNA is extracted from recovered Candida cells and subjected to microarray analysis.
Kruppa, Michael D.. 2009. Candida Albicans Gene Expression in an in Vivo Infection Model. Methods in Molecular Biology. Vol.499 77-83. https://doi.org/10.1007/978-1-60327-151-6_9 PMID: 19152041 ISSN: 1064-3745 ISBN: 9781588297600