Title
Effect of Polypeptide From Chlamys Farreri on UVB-Induced ROS/NF-κB/COX-2 Activation and Apoptosis in HaCaT Cells
Document Type
Article
Publication Date
8-3-2009
Description
Polypeptide from Chlamys farreri (PCF) is a novel marine polypeptide compound isolated from gonochoric Chinese scallop Chlamys farreri, this study we further investigate the mechanisms of PCF exerting its anti-apoptotic effect. The results indicated that PCF, ROS scavenger NAC and NF-κB inhibitor MG132 effectively inhibited UVB-induced HaCaT cells apoptosis. PCF (2.84 mM) showed potential ROS scavenging activities in a kinetic process. PCF (1.42-5.69 mM) dose-dependently increased the expressions of Cu, Zn-SOD, CAT and GPx meanwhile decreased the expressions of p-NF-κB/p65 and COX-2 in UVB-induced HaCaT cells. Additionally, pretreatment with NAC significantly declined the generation of ROS and the expression of p-NF-κB/p65. We concluded that ROS, NF-κB and COX-2 are involved in UVB-induced HaCaT cells apoptosis, PCF exerts its protective effects via scavenging ROS, increasing the expression of antioxidative enzymes and inhibition the activation of NF-κB and COX-2.
Citation Information
Liu, Xiao J.; Shi, Shao T.; Ye, Jun L.; Liu, Le Q.; Sun, Mi; and Wang, Chun Bo. 2009. Effect of Polypeptide From Chlamys Farreri on UVB-Induced ROS/NF-κB/COX-2 Activation and Apoptosis in HaCaT Cells. Journal of Photochemistry and Photobiology B: Biology. Vol.96(2). 109-116. https://doi.org/10.1016/j.jphotobiol.2009.04.010 PMID: 19481953 ISSN: 1011-1344