A Specific Molecular Beacon Probe for the Detection of Human Prostate Cancer Cells
The small-molecule, water-soluble molecular beacon probe 1 is hydrolyzed by the lysate and living cells of human prostate cancer cell lines (LNCaP), resulting in strong green fluorescence. In contrast, probe 1 does not undergo significant hydrolysis in either the lysate or living cells of human nontumorigenic prostate cells (RWPE-1). These results, corroborated by UV-Vis spectroscopy and fluorescent microscopy, reveal that probe 1 is a sensitive and specific fluorogenic and chromogenic sensor for the detection of human prostate cancer cells among nontumorigenic prostate cells and that carboxylesterase activity is a specific biomarker for human prostate cancer cells.
Jiang, Yu Lin; McGoldrick, Christopher A.; Yin, Deling; Zhao, Jing; Patel, Vini; Brannon, Marianne F.; Lightner, Janet W.; Krishnan, Koyamangalath; and Stone, William L.. 2012. A Specific Molecular Beacon Probe for the Detection of Human Prostate Cancer Cells. Bioorganic and Medicinal Chemistry Letters. Vol.22(11). 3632-3638. https://doi.org/10.1016/j.bmcl.2012.04.055 PMID: 22572577 ISSN: 0960-894X