MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
W. Scott Champney
John J. Laffan, Mitchell E. Robinson
Antibacterial agents specific for the 50S ribosomal subunit not only inhibit translation but also prevent assembly of that subunit. I examined the 30S ribosomal subunit in growing Escherichia coli cells to see if antibiotics specific for that subunit also had a second inhibitory effect. I used the aminoglycoside antibiotics paromomycin and neomycin, which bind specifically to the 30S ribosomal subunit. Both antibiotics inhibited the growth rate, viable cell number, and protein synthesis. I used a 3H-uridine pulse and chase assay to examine the kinetics of ribosome subunit assembly in the presence and absence of each antibiotic. Analysis revealed a concentration dependent inhibition of 30S subunit formation in the presence of each antibiotic. Sucrose gradient profiles of cell lysates showed the accumulation of an intermediate 21S translational particle. Taken together this data gives the first demonstration that 30S ribosomal subunit inhibitors can also prevent assembly of the small subunit.
Thesis - Open Access
Mehta, Roopal Manoj, "30S Ribosomal Subunit Assembly is a Target for Inhibition by Aminoglycoside Antibiotics in Escherichia coli." (2002). Electronic Theses and Dissertations. Paper 649. https://dc.etsu.edu/etd/649
Copyright by the authors.