MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Bert Lampson, Christopher Pritchett
Acinetobacter baumannii is an increasingly multidrug-resistant pathogen contributing to hospital-acquired infections necessitating the discovery of novel treatments. A bacterial second messenger, cyclic diguanosine monophosphate (cyclic di-GMP), can regulate various persistence factors that are potentially advantageous for survival in hospital environments. Cyclic di-GMP–modulating enzymes and cyclic di-GMP–binding effectors predictively are encoded in the Acinetobacter baumannii genome. I hypothesized that cyclic di-GMP controls motility, biofilm formation, and desiccation tolerance in Acinetobacter baumannii. Disrupting cyclic di-GMP–modulating enzymes or cyclic di-GMP–binding effectors should alter the regulatory effectiveness of these phenotypes. I tested the multidrug-resistant isolate Acinetobacter baumannii strain AB5075 and identified several transposon mutants that altered twitching motility, biofilm formation, and desiccation tolerance; these results suggest that cyclic di-GMP plays a role during these three responses in Acinetobacter baumannii AB5075. Inhibiting these cyclic di-GMP signaling pathways could produce novel mechanisms to combat this pathogen in the hospital environment.
Thesis - embargo
Reynolds, Garrett, "Cyclic di-GMP Regulates Motility, Biofilm Formation, and Desiccation Tolerance in Acinetobacter baumannii" (2022). Electronic Theses and Dissertations. Paper 4116. https://dc.etsu.edu/etd/4116
Copyright by the authors.
Available for download on Friday, September 15, 2023