MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Patrick Bradshaw, Cerrone Foster
Hypocretin/Orexin (HCRT) neurotransmission facilitates drug-seeking behavior. HCRT neurotransmission at HCRT-receptors 1 and 2 (HCRT-R1 and -R2, respectively) is implicated in addiction. During the shift to alcohol-dependency, adaptations in neurotransmitter systems occur in reward- and stress-related brain regions. Specifically, neurotransmission systems in the central amygdala (CeA) are modulated by alcohol drinking/exposure. Therefore, this study investigated Hcrtr1 and Hcrtr2 mRNA expression in the CeA of alcohol-dependent rats and in non-dependent controls during acute alcohol withdrawal. Fos mRNA expression in the CeA of alcohol-dependent and non-dependent rats was also determined to assess adaptations in neuronal activation. To our knowledge, this is the first study to utilize RNAscope to quantify Hcrtr1 and Hcrtr2 mRNA in a rodent model of alcohol dependence. However, Hcrtr1, Hcrtr2, and Fos mRNA levels were not found to be significantly different in alcohol-dependent rats compared to non-dependent controls, possibly due to the temporal dynamics of these neuroadaptations.
Thesis - unrestricted
Aldridge, Gabriel, "Hypocretin-Receptor mRNA Expression in the Central Amygdala of Alcohol-Dependent and Non-Dependent Rats" (2022). Electronic Theses and Dissertations. Paper 4047. https://dc.etsu.edu/etd/4047
Copyright by the authors.