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MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Hugh A. Miller III
Carolyn M. Wetzel, Cecilia A. McIntosh
Searches for sequence similarity against a previously isolated and known ABA regulated partial geranium cDNA on GenBank and TIGR databases gave results indicating sequence similarities to both firefly luciferase and 4-coumarate:CoA Ligase (4-CL). To determine whether or not this geranium cDNA, and a root mRNA from Arabidopsis that hybridized with the geranium cDNA, is 4-CL, specific Arabidopsis 4-CL primers were used for expression analysis by RT-PCR of Arabidopsis 4-CL mRNA from Arabidopsis root tissue. The expression pattern of the Arabidopsis 4-CL mRNA was identical with the expression pattern of the mRNA that hybridized with the geranium cDNA. This matching expression pattern gives very strong evidence that our geranium cDNA is 4-CL like and that Arabidopsis 4-CL is downregulated during water stress by ABA. This is significant in that it has not previously been reported. Northern analysis and PCR product sequence analysis further confirmed this expression data.
Thesis - restricted
Ashley, David W., "Characterization of a Novel Water Stress Related Protein from Geranium (Pelargonium hortorum)." (2001). Electronic Theses and Dissertations. Paper 39. https://dc.etsu.edu/etd/39
Copyright by the authors.