MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Ranjan Chakraborty, Gerardo Arcéo-Gomez
SABP2-interacting protein 470 (SIP470), a non-specific lipid transfer protein (nsLTP), was discovered in a yeast two-hybrid screening using SABP2 as bait and tobacco leaf proteins as prey. SABP2 is an important enzyme in systemic acquired resistance that converts salicylic acid to methyl salicylate. Localization studies are an important aspect to understanding the biological function of proteins. nsLTPs are generally considered apoplastic proteins and has been localized intracellularly and extracellularly. Transient expression shows highest expression of SIP470-eGFP at 2 days post infiltration into Nicotiana benthamiana. Confocal microscopy showed localization near the periphery of the cell. Subcellular localization using differential centrifugation showed that SIP470 is localized in the mitochondria. Mitochondria membranes are rich in lipids and have shown lipid exchange with the endoplasmic reticulum in mammalian systems. Co-localization of SIP470-eGFP+mCherry did not express complete co-localization in the targeted organelles. Co-localization pattern suggests possible localization in the endoplasmic reticulum.
Thesis - Withheld
Andrews, Shantaya, "Localization of SIP470, a Plant Lipid Transfer Protein in Nicotiana tabacum" (2018). Electronic Theses and Dissertations. Paper 3520. https://dc.etsu.edu/etd/3520
Copyright by the authors.
Available for download on Saturday, November 12, 2022