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MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Karl Joplin, Christopher Pritchett
The morphogenesis of C. albicans is a major aspect of its virulence and is regulated by quorum sensing (QS) molecules they produce, as well as the presence of neighboring microbes.Two mutant transporters, SSU1 and CDR4, were characterized for their ability to form biofilms in the presence of cyclic-di-GMP and 3-oxo-12-homoserine lactone. While homoserine lactone showed a decrease in biofilm density of both mutants compared to the wild-type strain, wild-type and ssu1 biofilm densities increased considerably in the presence of cyclic-di-GMP while testing lower inocula. Additionally, it has been shown that C. albicans mutants lacking the hybrid histidine kinase, Chk1, are refractory to the effects of farnesol, a QS molecule that inhibits morphogenesis.We determined both CDR4 and SSU1 expression is reduced or highly repressed in the chk1, ypd1, and skn7 null strains. Our results suggest these two genes are downstream targets in a pathway regulated by Chk1p.
Thesis - restricted
Stuffle, Derek, "Two Component Pathway Regulation of Transport Genes Involved in Quorum Sensing and Response to Bacterial Signaling Molecules in C. albicans" (2018). Electronic Theses and Dissertations. Paper 3373. https://dc.etsu.edu/etd/3373
Copyright by the authors.