MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Cecilia McIntosh, Cerrone Foster
N-acylethanolamines (NAEs) are derived from a minor membrane lipid constituent N-acylphosphatidylethanolamine and are hydrolyzed by fatty acid amide hydrolases (FAAH) into free fatty acid (FFA) and ethanolamine in both plants and animals. In Arabidopsis, NAE plays an important physiological role in growth/development and response to stress. Although NAEs are reported in tomato, their metabolic pathway remains undiscovered. It is hypothesized that there is a functional FAAH in tomato that hydrolyzes NAEs. To this extent, a putative gene that likely encodes for putative SlFAAH1 protein was identified, cloned, and heterologously expressed. Amidase activity was tested using radiolabeled NAE substrates. Furthermore, expression of putative SlFAAH1 transcripts and protein activity was quantified at different developmental stages to demonstrate endogenous amidase activity in tomato seedlings. In future, molecular and biochemical characterization of tomato FAAH will further test the conserved nature of NAE metabolic pathway in plants.
Thesis - Open Access
Tiwari, Vijay, "Isolation and Heterologous Expression of Putative Tomato Fatty Acid Amide Hydrolase" (2016). Electronic Theses and Dissertations. Paper 3138. https://dc.etsu.edu/etd/3138
Copyright by the authors.