Characterization of SIP470, a Family 1 Lipid Transfer Protein and its Role in Plant Stress Signaling
Degree Name
MS (Master of Science)
Program
Biology
Date of Award
8-2016
Committee Chair or Co-Chairs
Dhirendra Kumar
Committee Members
Ranjan Chakraborty, Karl Joplin
Abstract
SIP470, a putative tobacco lipid transfer protein, was identified in a yeast two-hybrid screen to interact with SABP2. SABP2 is a critical role in SA-mediated signaling in tobacco and other plants. In vitro studies using purified recombinant SIP470 confirmed that it is a lipid binding protein. In an attempt to determine its role in mediating stress responses, Arabidopsis T-DNA insertion knockout lines lacking SIP470 homolog were used for the analysis. These mutant plants were defective in basal resistance against microbial pathogens. Expression of defense gene PR-1 was also delayed in these mutant plants. Interestingly, these mutant plants were not defective in inducing systemic acquired resistance. Besides biotic stress, these mutant plants also showed increased susceptibility to abiotic stresses. To directly study the role of SIP470 in tobacco plants, transgenic tobacco lines, with reduced levels of SIP470 expression, were generated using RNAi and transgenic lines overexpressing SIP470 were also generated.
Document Type
Thesis - unrestricted
Recommended Citation
Audam, Timothy Ndagi, "Characterization of SIP470, a Family 1 Lipid Transfer Protein and its Role in Plant Stress Signaling" (2016). Electronic Theses and Dissertations. Paper 3103. https://dc.etsu.edu/etd/3103
Copyright
Copyright by the authors.
Included in
Biology Commons, Molecular Biology Commons, Plant Biology Commons, Plant Pathology Commons