Understanding the Role of SABP2-Interacting Protein (SIP)-432: a Premnaspirodiene Oxygenase Enzyme In Stress Signaling
Abstract
Adverse environmental conditions have a significant negative impact on crop yields. The increased temperature and drought have caused a decrease in plant survival and an increase in plant pathogens that also affect plant survival. Several known plant proteins contribute to pathogen resistance and environmental stressors, but the overall metabolic pathway remains unknown. By piecing together important genes/proteins in plant immune response, the defense mechanisms can be better understood. This study focuses on the characterization of a SIP432 (SABP2-interacting protein). SIP432 is a premnaspirodiene oxygenase-like enzyme. SABP2 is a critical component of salicylic acid-mediated plant immunity. Premnaspirodiene, a sesquiterpene (15 carbon) can be converted into an antimicrobial compound/s by a hydroxylation reaction in plants. The interaction of SIP432 with SABP2 implies a role for SIP432 in plant defense signaling. To study the role of SIP432 in abiotic and biotic stress responses, we are using the Arabidopsis thaliana SIP432 knockout mutants. Wild-type and SIP432 mutant Arabidopsis plants will be subjected to abiotic stress (salt and drought) and also challenged with plant pathogens (Pseudomonas syringae) to understand its role in stress signaling pathways. All this will allow for the discovery of signaling in plant-stress response. Discovering the function of this protein could help uncover the stress response pathway/s in plants.
Start Time
16-4-2025 10:00 AM
End Time
16-4-2025 11:00 AM
Room Number
219
Presentation Type
Oral Presentation
Presentation Subtype
UG Orals
Presentation Category
Health and STEM
Faculty Mentor
Dhirendra Kumar
Understanding the Role of SABP2-Interacting Protein (SIP)-432: a Premnaspirodiene Oxygenase Enzyme In Stress Signaling
219
Adverse environmental conditions have a significant negative impact on crop yields. The increased temperature and drought have caused a decrease in plant survival and an increase in plant pathogens that also affect plant survival. Several known plant proteins contribute to pathogen resistance and environmental stressors, but the overall metabolic pathway remains unknown. By piecing together important genes/proteins in plant immune response, the defense mechanisms can be better understood. This study focuses on the characterization of a SIP432 (SABP2-interacting protein). SIP432 is a premnaspirodiene oxygenase-like enzyme. SABP2 is a critical component of salicylic acid-mediated plant immunity. Premnaspirodiene, a sesquiterpene (15 carbon) can be converted into an antimicrobial compound/s by a hydroxylation reaction in plants. The interaction of SIP432 with SABP2 implies a role for SIP432 in plant defense signaling. To study the role of SIP432 in abiotic and biotic stress responses, we are using the Arabidopsis thaliana SIP432 knockout mutants. Wild-type and SIP432 mutant Arabidopsis plants will be subjected to abiotic stress (salt and drought) and also challenged with plant pathogens (Pseudomonas syringae) to understand its role in stress signaling pathways. All this will allow for the discovery of signaling in plant-stress response. Discovering the function of this protein could help uncover the stress response pathway/s in plants.