Isolation and characterization of siderophore(s) from Pseudomonas mosselii
Abstract
Iron is essential for growth of most microorganisms with a few exceptions. It functions as an important cofactor in many important biological processes. However, abundant iron found in soil is rendered insoluble and is unavailable to microorganisms for use. To overcome this restriction, many bacterial species produce siderophores, a small secondary metabolite that has high affinity for iron. Siderophores allow for bacterial cells to obtain otherwise insoluble iron. Pseudomonas mosselii, a gram negative, aerobic and motile bacterium was isolated from a local soil sample. P. mosselii is known to be an opportunistic pathogen & has been reported to have antimicrobial activities against plant pathogens. P. mosselii was found to produce siderophore(s) under an iron restricted environment, however, they have not been structurally characterized. Therefore, it was of our interest to isolate, purify and structurally characterize the siderophore produced by P, mosselii. Our preliminary chemical characterization indicates a catechol type fluorescent compound most likely belonging to pyoverdine family. It was also found to have a strong antimicrobial activity against gram positive bacterium. At present, further purification and structural characterizations are underway in our laboratory.
Start Time
16-4-2025 1:30 PM
End Time
16-4-2025 4:00 PM
Presentation Type
Poster
Presentation Category
Science, Technology and Engineering
Student Type
Graduate Student - Masters
Faculty Mentor
Dr. Ranjan Chakraborty
Faculty Department
Biomedical Health Sciences
Isolation and characterization of siderophore(s) from Pseudomonas mosselii
Iron is essential for growth of most microorganisms with a few exceptions. It functions as an important cofactor in many important biological processes. However, abundant iron found in soil is rendered insoluble and is unavailable to microorganisms for use. To overcome this restriction, many bacterial species produce siderophores, a small secondary metabolite that has high affinity for iron. Siderophores allow for bacterial cells to obtain otherwise insoluble iron. Pseudomonas mosselii, a gram negative, aerobic and motile bacterium was isolated from a local soil sample. P. mosselii is known to be an opportunistic pathogen & has been reported to have antimicrobial activities against plant pathogens. P. mosselii was found to produce siderophore(s) under an iron restricted environment, however, they have not been structurally characterized. Therefore, it was of our interest to isolate, purify and structurally characterize the siderophore produced by P, mosselii. Our preliminary chemical characterization indicates a catechol type fluorescent compound most likely belonging to pyoverdine family. It was also found to have a strong antimicrobial activity against gram positive bacterium. At present, further purification and structural characterizations are underway in our laboratory.