Project Title

Extraction and Partial Purification of an Antibiotic-like Compound From the Soil Bacterium Rhoddococcus Strain KCHXC3

Authors' Affiliations

Elizabeth Bond, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN. Abbas Shilabin, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN. Bert Lampson, Department of Health Sciences, College of Public Health, East Tennessee State University, Johnson City, TN.

Location

Culp Room 210

Start Date

4-6-2022 10:15 AM

End Date

4-6-2022 10:30 AM

Faculty Sponsor’s Department

Chemistry

Name of Project's Faculty Sponsor

Abbas Shilabin

Classification of First Author

Undergraduate Student

Competition Type

Non-Competitive

Type

Boland Symposium

Project's Category

Chromatography

Abstract or Artist's Statement

Rhodococcus bacteria have many secondary metabolic pathways that may produce novel natural products. The bacterium Rhodococcus strain KCHXC3 was isolated from a soil sample collected near Kingsport, Tennessee and was found to produce an inhibitory compound active against a broad array of bacterial species, including the Gram negative pathogen Shigella. The aim of this research is to extract and purify the compound for future structure elucidation. A mixture of compounds from 3 month old agar plates inoculated with strain KCHXC3 was extracted using ethyl acetate. The crude extract was then partially purified utilizing a Sephadex LH-20 column, followed by an analytical NH2 HPLC column. This purification resulted in a dried crystalline-like active compound that is white in color and needle-like in shape. Structural studies such as NMR and GC-MS were performed in order to determine the purity of this solid.

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Apr 6th, 10:15 AM Apr 6th, 10:30 AM

Extraction and Partial Purification of an Antibiotic-like Compound From the Soil Bacterium Rhoddococcus Strain KCHXC3

Culp Room 210

Rhodococcus bacteria have many secondary metabolic pathways that may produce novel natural products. The bacterium Rhodococcus strain KCHXC3 was isolated from a soil sample collected near Kingsport, Tennessee and was found to produce an inhibitory compound active against a broad array of bacterial species, including the Gram negative pathogen Shigella. The aim of this research is to extract and purify the compound for future structure elucidation. A mixture of compounds from 3 month old agar plates inoculated with strain KCHXC3 was extracted using ethyl acetate. The crude extract was then partially purified utilizing a Sephadex LH-20 column, followed by an analytical NH2 HPLC column. This purification resulted in a dried crystalline-like active compound that is white in color and needle-like in shape. Structural studies such as NMR and GC-MS were performed in order to determine the purity of this solid.