Project Title

Extraction, Purification, and Characterization of Radioprotective Agent gamma-Tocotrienol Isomer in Palm Oil

Authors' Affiliations

Kwabena Fobi, Department of Chemistry, College of Arts and Science, East Tennessee State University, Johnson City, TN Bronson Lynn, Department of Chemistry, College of Arts and Science, East Tennessee State University, Johnson City, TN Abbas Gholipour Shilabin, Department of Chemistry, College of Arts and Science, East Tennessee State University, Johnson City, TN

Location

Ballroom

Start Date

4-12-2019 9:00 AM

End Date

4-12-2019 2:30 PM

Poster Number

46

Faculty Sponsor’s Department

Chemistry

Name of Project's Faculty Sponsor

Dr. Abbas Gholipour Shilabin

Type

Poster: Competitive

Classification of First Author

Graduate Student-Master’s

Project's Category

Biological and Chemical Sciences, Healthcare and Medicine

Abstract Text

The clinical consequences of ionizing radiation exposure remain one of the leading causes of death in the United States. Much research has been carried out to discover a potential countermeasure for acute radiation syndrome (ARS) without success. The United States Food and Drug Administration (US FDA) has not accepted any effective and harmless ionizing radiation therapy agents (radioprotectors) for treating ARS. It has recently been discovered that g-tocotrienol (GT-3), one of the E vitamers chiefly present in palm oil, has radioprotective abilities in mice and nonhuman primate (NHP) models. Though GT-3 is one of the most promising countermeasures discovered, the separation and purification from other vitamers or its matrix is difficult. This has limited its characterization, derivatization, and biomedical application. We have therefore designed novel chromatographic methods to optimize separation and purification. Thin layer chromatography (TLC) was used to ascertain the best solvent system for column chromatography (CC). Exactly 8% ethyl acetate in hexane employed in TLC and CC resulted in good separation (Rf ≥ 0.3) and purification. Various fractions presumed to contain GT-3 were collected and analyzed to confirm the exact structure using 1H NMR, 13C NMR, DEPT, and GC-MS. Results obtained so far have revealed the exact structure of the compound. However, some traces of impurities have been indicated by the NMR outcomes; therefore, high-performance liquid chromatography (HPLC) will be used to maximize GT-3 purification. This present study will be instrumental in elucidating the biochemical structure of various complex plant bioactive components that are hard to isolate and analyze. It is envisioned that this work will help to erase the knowledge deficit in medicinal chemistry and assist in the development of new medications for ARS.

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Apr 12th, 9:00 AM Apr 12th, 2:30 PM

Extraction, Purification, and Characterization of Radioprotective Agent gamma-Tocotrienol Isomer in Palm Oil

Ballroom

The clinical consequences of ionizing radiation exposure remain one of the leading causes of death in the United States. Much research has been carried out to discover a potential countermeasure for acute radiation syndrome (ARS) without success. The United States Food and Drug Administration (US FDA) has not accepted any effective and harmless ionizing radiation therapy agents (radioprotectors) for treating ARS. It has recently been discovered that g-tocotrienol (GT-3), one of the E vitamers chiefly present in palm oil, has radioprotective abilities in mice and nonhuman primate (NHP) models. Though GT-3 is one of the most promising countermeasures discovered, the separation and purification from other vitamers or its matrix is difficult. This has limited its characterization, derivatization, and biomedical application. We have therefore designed novel chromatographic methods to optimize separation and purification. Thin layer chromatography (TLC) was used to ascertain the best solvent system for column chromatography (CC). Exactly 8% ethyl acetate in hexane employed in TLC and CC resulted in good separation (Rf ≥ 0.3) and purification. Various fractions presumed to contain GT-3 were collected and analyzed to confirm the exact structure using 1H NMR, 13C NMR, DEPT, and GC-MS. Results obtained so far have revealed the exact structure of the compound. However, some traces of impurities have been indicated by the NMR outcomes; therefore, high-performance liquid chromatography (HPLC) will be used to maximize GT-3 purification. This present study will be instrumental in elucidating the biochemical structure of various complex plant bioactive components that are hard to isolate and analyze. It is envisioned that this work will help to erase the knowledge deficit in medicinal chemistry and assist in the development of new medications for ARS.