Project Title

Theranostic Nanoparticles for Simultanous Detection and Treatment of Cancer

Author Names

Samson Niyi DadaFollow

Authors' Affiliations

Samson Dada, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN

Location

Ballroom

Start Date

4-12-2019 9:00 AM

End Date

4-12-2019 2:30 PM

Poster Number

42

Faculty Sponsor’s Department

Chemistry

Name of Project's Faculty Sponsor

Dr. Hua Mei

Type

Poster: Competitive

Classification of First Author

Graduate Student-Master’s

Project's Category

Organic Chemistry

Abstract Text

Abstract

Samson Dada and Dr. Hua Mei, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN

Our overall research goal is to synthesize a water-soluble, bio-absorbable theranostic nanoparticle (NP) that will improve diagnostic and therapeutic efficacy for cancer. Such theranostic nanoparticles are composed of carbon dots (CDs), conjugated with a targeting agent through a non-cleavable peptide bond; and an anticancer drug Doxorubicin (DOX) using an acid-labile hydrazine linkage for targeted delivery and bio-imaging functions. Recent studies have shown that Carbon dots (CDs) are of interest in biological applications due to their unique properties such as inherent fluorescence, extremely high biocompatibility, and facile synthetic route. The large surface area and multiple surface functionalities make CDs versatile platforms to conjugate with other moieties, including therapeutic agents or targeting agents. The target agents, such as folic acid (FA), are proposed to be permanently linked with CDs to improve the target specificity of the tumor cells. Folic acid is used as a targeting agent as it is a water-soluble, low molecular weight vitamin as it plays an essential role in cell survival and binds with high affinity to the folate receptor (FR) – a membrane-anchored protein that is a cancer biomarker. The multimodal nano-platforms of CDs can also facilitate the delivery the anticancer drugs. The anticancer drug is attached by a cleavable linker that can release the drug inside the tumor cell. We will use the cytotoxic chemotherapeutic agent doxorubicin (DOX) as an example. One series of CDs, FA-CD and FA-CD-DOX, are successfully prepared in the lab. The UV-vis and Fluorescence spectra of the sample was investigated and compared. The concentration of each part in nanoparticles are calculated. The final Drug Load Content (DLC) and Drug Load Efficiency (DLE) are also calculated and compared with the literature. Another series of FA-CD-DOX will be prepared and compared. The characterization of the diagnostic and therapeutic potential of the NP particles will be carried out in the pharmaceutical department.

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Apr 12th, 9:00 AM Apr 12th, 2:30 PM

Theranostic Nanoparticles for Simultanous Detection and Treatment of Cancer

Ballroom

Abstract

Samson Dada and Dr. Hua Mei, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN

Our overall research goal is to synthesize a water-soluble, bio-absorbable theranostic nanoparticle (NP) that will improve diagnostic and therapeutic efficacy for cancer. Such theranostic nanoparticles are composed of carbon dots (CDs), conjugated with a targeting agent through a non-cleavable peptide bond; and an anticancer drug Doxorubicin (DOX) using an acid-labile hydrazine linkage for targeted delivery and bio-imaging functions. Recent studies have shown that Carbon dots (CDs) are of interest in biological applications due to their unique properties such as inherent fluorescence, extremely high biocompatibility, and facile synthetic route. The large surface area and multiple surface functionalities make CDs versatile platforms to conjugate with other moieties, including therapeutic agents or targeting agents. The target agents, such as folic acid (FA), are proposed to be permanently linked with CDs to improve the target specificity of the tumor cells. Folic acid is used as a targeting agent as it is a water-soluble, low molecular weight vitamin as it plays an essential role in cell survival and binds with high affinity to the folate receptor (FR) – a membrane-anchored protein that is a cancer biomarker. The multimodal nano-platforms of CDs can also facilitate the delivery the anticancer drugs. The anticancer drug is attached by a cleavable linker that can release the drug inside the tumor cell. We will use the cytotoxic chemotherapeutic agent doxorubicin (DOX) as an example. One series of CDs, FA-CD and FA-CD-DOX, are successfully prepared in the lab. The UV-vis and Fluorescence spectra of the sample was investigated and compared. The concentration of each part in nanoparticles are calculated. The final Drug Load Content (DLC) and Drug Load Efficiency (DLE) are also calculated and compared with the literature. Another series of FA-CD-DOX will be prepared and compared. The characterization of the diagnostic and therapeutic potential of the NP particles will be carried out in the pharmaceutical department.