Project Title

Simple Pretreatment of Arundo Donax and Enzymatic Conversion of Cellulosic Materials to Glucose

Authors' Affiliations

Akintayo Fatunwase, Department of Chemistry, East Tennessee State University, Johnson city, TN.

Location

AUDITORIUM ROOM 137A

Start Date

4-12-2019 2:40 PM

End Date

4-12-2019 2:55 PM

Faculty Sponsor’s Department

Chemistry

Type

Oral Presentation

Classification of First Author

Graduate Student-Master’s

Project's Category

Analytical Chemistry

Project's Category

Arts and Humanities

Abstract Text

Arundodonax (Giant reed Plant) contains cellulose, hemicellulose and lignin and considered as a biomass resources for biofuels. Cellulose is a polymer of several d-glucose linked units coupled with beta-1, 4 glycosidic bonds. The lignin must be broken down to obtain cellulose.Brown and white rot fungusbreak down lignin through a fenton mechanism using hydroxyl radicals. Current work explores degradation of cellulose byisolating microbial communities followed by inoculating 1% carboxymethyl cellulose (CMC) or arundodonax in nutrient media. The microbes demonstrate long-term viability using CMS or arundodonax the sole carbon source.Pretreatment with microbes result in enhanced enzymatic hydrolysis at 50 °C using commercial cellulase over time. The simple dinitrosalicylic acid assay method quantifies glucose, the main product of enzymatic hydrolysis.

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Apr 12th, 2:40 PM Apr 12th, 2:55 PM

Simple Pretreatment of Arundo Donax and Enzymatic Conversion of Cellulosic Materials to Glucose

AUDITORIUM ROOM 137A

Arundodonax (Giant reed Plant) contains cellulose, hemicellulose and lignin and considered as a biomass resources for biofuels. Cellulose is a polymer of several d-glucose linked units coupled with beta-1, 4 glycosidic bonds. The lignin must be broken down to obtain cellulose.Brown and white rot fungusbreak down lignin through a fenton mechanism using hydroxyl radicals. Current work explores degradation of cellulose byisolating microbial communities followed by inoculating 1% carboxymethyl cellulose (CMC) or arundodonax in nutrient media. The microbes demonstrate long-term viability using CMS or arundodonax the sole carbon source.Pretreatment with microbes result in enhanced enzymatic hydrolysis at 50 °C using commercial cellulase over time. The simple dinitrosalicylic acid assay method quantifies glucose, the main product of enzymatic hydrolysis.