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MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
John J. Laffan
Thomas W. Ecay Jr., Robert V. Schoborg, Brian P. Rowe
Bacillus anthracis is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of B. anthracis is poorly understood. B. anthracis is one of many biological weapons used today. A -/- mutant strain of B. anthracis that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The dnaB gene, essential for B. subtilis initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of dnaB to study the genetics of B. anthracis. An electro-transformation system was formulated to knock-out the -/- B. anthracis dnaB gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of B. anthracis. We also have formulated an electro-transformation system and vector system for use in B. anthracis.
Thesis - Campus Only
Anderson, Robert Clayton III, "Creating Genetic Engineering Tools for Investigating Bacillus anthracis." (2003). Electronic Theses and Dissertations. Paper 819. http://dc.etsu.edu/etd/819
Copyright by the authors.