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Degree Name
MS (Master of Science)
Program
Biology
Date of Award
12-2001
Committee Chair or Co-Chairs
Hugh A. Miller III
Committee Members
Carolyn M. Wetzel, Cecilia A. McIntosh
Abstract
Searches for sequence similarity against a previously isolated and known ABA regulated partial geranium cDNA on GenBank and TIGR databases gave results indicating sequence similarities to both firefly luciferase and 4-coumarate:CoA Ligase (4-CL). To determine whether or not this geranium cDNA, and a root mRNA from Arabidopsis that hybridized with the geranium cDNA, is 4-CL, specific Arabidopsis 4-CL primers were used for expression analysis by RT-PCR of Arabidopsis 4-CL mRNA from Arabidopsis root tissue. The expression pattern of the Arabidopsis 4-CL mRNA was identical with the expression pattern of the mRNA that hybridized with the geranium cDNA. This matching expression pattern gives very strong evidence that our geranium cDNA is 4-CL like and that Arabidopsis 4-CL is downregulated during water stress by ABA. This is significant in that it has not previously been reported. Northern analysis and PCR product sequence analysis further confirmed this expression data.
Document Type
Thesis - restricted
Recommended Citation
Ashley, David W., "Characterization of a Novel Water Stress Related Protein from Geranium (Pelargonium hortorum)." (2001). Electronic Theses and Dissertations. Paper 39. https://dc.etsu.edu/etd/39
Copyright
Copyright by the authors.