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Degree Name
MS (Master of Science)
Program
Biology
Date of Award
8-2017
Committee Chair or Co-Chairs
Dennis Defoe
Committee Members
Tom W. Ecay, Cecilia McIntosh
Abstract
Corneal endothelial cells have been shown to possess a uniform polygonal and complex multipolar shape at their apical and basolateral surface respectively. We established a morphological timetable to study how this complex shape arises postnatally. Corneas were collected from mice between postnatal day 8 to postnatal day 35 and labelled with antibodies specific for ZO-1 and NCAM at apical and basolateral region, respectively. Images were collected using wide-field fluorescence microscopy and morphometrically analyzed. Results showed that apical cell sizes increase linearly over the first 3 weeks, plateauing at 4-5 weeks postnatally with increased regularity. Basolateral membrane surfaces remained relatively smooth prior to eyelid opening and thereafter begins developing showing differences in development from periphery to the center until about 4 weeks postnatally when the wavy processes get vivid. Results indicate concurrent and independent development at both poles of the corneal endothelium, with more complexity seen at the basolateral surface.
Document Type
Thesis - restricted
Recommended Citation
Ojo, Victor Temidayo, "Postnatal Cell Shape development of the Corneal Endothelium in Mice" (2017). Electronic Theses and Dissertations. Paper 3301. https://dc.etsu.edu/etd/3301
Copyright
Copyright by the authors.