Degree Name

MS (Master of Science)

Program

Biology

Date of Award

5-2016

Committee Chair or Co-Chairs

Hugh Miller

Committee Members

Aruna Kilaru, Leonard Robertson

Abstract

Phylloquinone is a natural analog of vitamin K that has been shown to both inhibit cancer cell growth and induce apoptosis in several cancer cell lines. This study examined these effects in a non-Hodgkin lymphoma cell line, known as U937. Cell growth inhibition and apoptosis were assessed through the quantification of cell density and area, following treatment with several concentrations of phylloquinone. In addition, apoptosis was detected and quantified using immunofluorescent markers of apoptosis (i.e. annexin V, APO-BrdU). Treatment with phylloquinone resulted in reduced overall cell density, increased overall cell area, and an increased frequency of apoptosis in U937 cells. Increasing both phylloquinone concentration and treatment time enhanced these effects. These results are significant because they document the anti-cancer effects of this analog of vitamin K, as well as provide insight into the morphological changes that occur during apoptosis in U937 cells.

Document Type

Thesis - Open Access

Copyright

Copyright by the authors.

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