Degree Name

MS (Master of Science)

Program

Biology

Date of Award

12-2015

Committee Chair or Co-Chairs

Edward M. Onyango

Committee Members

Eric Mustain, Jonathan Peterson, Mohamed Elgazzar, Effiong Otukonyong

Abstract

This study was performed to test the in vitro effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu”, a traditional Kenyan cultured milk, on 3T3-L1 and Caco-2 cell lines. Cultures of fully mature 3T3-L1 adipocytes were treated with bacterial isolate cell extract (CE), filtered spent broth (FSB) from overnight bacterial culture, or with a PBS control. Expression levels of PPAR³1 and 2, C/EBP±, and ATGL proteins in 3T3-L1 cells were upregulated by FSB treatment. CE treatment did not affect protein expression levels. Expression of MTTP and SREBP-1c proteins in Caco-2 cells showed no change with either treatment. Optical density measurements from Oil-Red-O stained 3T3-L1 adipocytes increased from PBS control cells to 25μl/ml FSB treated cells; measurements were reduced by treatments above 25μl/ml FSB. In conclusion, filtered spent broth prepared from a culture of Lactobacillus rhamnosus, isolated from “amabere amaruranu” cultured milk showed PPAR³1 and 2, C/EBP±, and ATGL agonistic properties.

Document Type

Thesis - Open Access

Copyright

Copyright by the authors.

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