MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Edward M. Onyango
Eric Mustain, Jonathan Peterson, Mohamed Elgazzar, Effiong Otukonyong
This study was performed to test the in vitro effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu”, a traditional Kenyan cultured milk, on 3T3-L1 and Caco-2 cell lines. Cultures of fully mature 3T3-L1 adipocytes were treated with bacterial isolate cell extract (CE), filtered spent broth (FSB) from overnight bacterial culture, or with a PBS control. Expression levels of PPAR³1 and 2, C/EBP±, and ATGL proteins in 3T3-L1 cells were upregulated by FSB treatment. CE treatment did not affect protein expression levels. Expression of MTTP and SREBP-1c proteins in Caco-2 cells showed no change with either treatment. Optical density measurements from Oil-Red-O stained 3T3-L1 adipocytes increased from PBS control cells to 25μl/ml FSB treated cells; measurements were reduced by treatments above 25μl/ml FSB. In conclusion, filtered spent broth prepared from a culture of Lactobacillus rhamnosus, isolated from “amabere amaruranu” cultured milk showed PPAR³1 and 2, C/EBP±, and ATGL agonistic properties.
Thesis - Open Access
Kotala, Justin E., "Influence of Lactobacillus rhamnosus Isolated from “Amabere Amaruranu” Cultured Milk on Adipogenesis" (2015). Electronic Theses and Dissertations. Paper 2608. http://dc.etsu.edu/etd/2608
Copyright by the authors.