MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Cassandra T. Eagle
Aleksey Vasiliev, Marina Roginskaya
β-glucan is a major component of the fungal cell wall consisting of (1→3)-β linked glucose polymers with (1→6)-β linked side chains. The published classical isolation procedure of β-glucan from Saccharomyces cerevisiae is expensive and time-consuming. Thus, the aim of this research was to develop an effective procedure for the extraction of glucans. We have developed a new method for glucan extraction that will be cost effective and will maintain the native structure of the glucan. The method that we developed is 80% faster and utilizes 1/3 of the reagents compared to the published classical method. Further, the method developed increases the yield from 2.9 % to 10.3 %. Our new process has a branching frequency of 18.4 down from 197 and a side chain of 5.1 up from 2.5. The data indicate a more preserved native structure of isolated glucans.
Thesis - Open Access
Asare, Shardrack O., "Optimized Acid/Base Extraction and Structural Characterization of β-glucan from Saccharomyces Cerevisiae" (2015). Electronic Theses and Dissertations. Paper 2513. http://dc.etsu.edu/etd/2513
Copyright by the authors.