Degree Name

MS (Master of Science)

Program

Biology

Date of Award

8-2011

Committee Chair or Co-Chairs

Dhirendra Kumar

Committee Members

Thomas F. Laughlin, Ranjan N. Chakraborty

Abstract

Salicylic acid binding protein 2 (SABP2) is one of the key enzymes in salicylic acid-dependent plant defense pathway. SABP2 is a 29 kDa protein present in extremely low abundance in plants and it catalyzes the conversion of signaling molecule methyl salicylate into salicylic acid. Although it has been shown that 6x His-tagged SABP2 over expressed in E. coli is a homodimer, its exact conformation in planta is still unknown. Therefore, we proposed to determine if SABP2 exist as a dimer and/or monomer under natural condition. To verify the exact conformation of native SABP2 protein in plant, SABP2 was purified from wild type tobacco using a 5-step purification protocol. Analysis of purified SABP2 in gel filtration and immunoblot assay suggested that SABP2 exists as a monomer in tobacco plant. Studies on SABP2 conformation will give us insight into the structure and functional relationship of this protein in salicylic acid-dependent disease resistance pathway.

Document Type

Thesis - Open Access

Copyright

Copyright by the authors.

Included in

Biology Commons

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