MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Karl H. Joplin
Dhirena Kumar, Hugh A. Miller III
The objective of this study was to identify genes that are differentially regulated during diapause when compared with nondiapausing pupae in Sarcophaga crassipalpis. The results of a Suppression Subtractive Hybridization procedure was used to indentify genes that are differentially regulated in both diapause and nondiapausing states while suppressing genes that are common to both states. Randomly picked colonies from both subtractive libraries were isolated and the inserts sequenced. The sequences were analyzed using the bioinformatics tools NCBI, BlastX, Clustal W, etc. Out of 384 clones, 59 genes were found to be upregulated during diapause and 37 genes were found to be upregulated during a nondiapause pupal stage, no genes were found to be expressed commonly in both the diapause and nondiapause constructed libraries.
Thesis - Open Access
Karki, Puja, "Differential Expression of Genes During Diapause in the Flesh Fly, Sarcophaga crassipalpis." (2009). Electronic Theses and Dissertations. Paper 1818. http://dc.etsu.edu/etd/1818
Copyright by the authors.