Degree Name

MS (Master of Science)

Program

Biology

Date of Award

8-2009

Committee Chair or Co-Chairs

Karl H. Joplin

Committee Members

Dhirena Kumar, Hugh A. Miller III

Abstract

The objective of this study was to identify genes that are differentially regulated during diapause when compared with nondiapausing pupae in Sarcophaga crassipalpis. The results of a Suppression Subtractive Hybridization procedure was used to indentify genes that are differentially regulated in both diapause and nondiapausing states while suppressing genes that are common to both states. Randomly picked colonies from both subtractive libraries were isolated and the inserts sequenced. The sequences were analyzed using the bioinformatics tools NCBI, BlastX, Clustal W, etc. Out of 384 clones, 59 genes were found to be upregulated during diapause and 37 genes were found to be upregulated during a nondiapause pupal stage, no genes were found to be expressed commonly in both the diapause and nondiapause constructed libraries.

Document Type

Thesis - Open Access

Copyright

Copyright by the authors.

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