MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Ningfeng (Peter) Zhao, Peng Sun
Much attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate (TMOS) as precursor, and at least 7 uses in the sol-gel induced electrochemically with tetraethyl orthosilicate (TEOS) as precursor. The sol-gel made with TEOS as precursor and HCl as catalyst showed stability in enzyme activity for 11 uses but the activity decreases on the same sol-gel when modified with silica nanoparticles. Factors such as optimal incubation time of glucose solution, the reproducibility between different sol-gels, and the storage time were investigated. Good linearity and analytical results on real samples were obtained. The detection method was based on a colorimetric method for determining the concentration of hydrogen peroxide produced from the oxidation reaction of glucose.
Thesis - Open Access
Hamdan, Suzana, "Studies of the Preparations and Use of Sol-Gel for Enzyme Immobilization and Analytical Applications." (2009). Electronic Theses and Dissertations. Paper 1802. http://dc.etsu.edu/etd/1802
Copyright by the authors.