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MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Cecilia A. McIntosh
Daniel K. Owens, Dhirena Kumar
Flavonoids are plant secondary metabolites well known for many key roles in the life cycle of plants. They also can affect human health. Citrus paradisi is known to produce several glucosylated flavonoids and these compounds are glucosylated by enzymes known as glucosyltransferases (GTs). The focus of this research was to optimize the heterologous expression, enrichment, and biochemical characterization of grapefruit putative GT protein, PGT2, and to test the hypothesis that PGT2 is a flavonoid GT. Results showed detectable amounts of activity with quercetin, a flavonol; however, activity was lower than what would be expected if this enzyme were a flavonol-specific GT. In an additional aspect of this study, bioinformatics were used to test the hypothesis that additional putative GT clones could be identified using the harvEST database.
Thesis - Campus Only
Mallampalli, Venkata K. P. S, "Expression and Biochemical Function of Putative Flavonoid GT Clones from Grapefruit and Identification of New Clones using the harvEST Database." (2009). Electronic Theses and Dissertations. Paper 1788. http://dc.etsu.edu/etd/1788
Copyright by the authors.