MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Andromeda Nauli, Ph.D.
Edward Onyango, Ph.D., Effiong Otukonyong, Ph.D.
Dietary fats are mainly transported by the intestine in lipoproteins: chylomicrons (CMs) and very low density lipoproteins (VLDLs). Unfortunately, studies of the intestinal absorption of dietary fat have been hampered by the lack of an adequate in vitro model system. As an in vitro model Caco-2 cells are able to secrete lipoproteins. We investigated the possible factors that may affect the secretion of CMs through the ultracentrifugation technique. The dose-dependent effects of oleic acid, mono-olein, egg lecithin, collagen matrix, and the effect of cell differentiation on CM secretion were then tested. We found that oleic acid, lecithin, and cell differentiation are critical for CM secretion by Caco-2 cells. To further confirm that our optimal condition is, in fact, favorable for efficient CM production, we compared it with control groups. We observed that our condition led to more efficient CM secretion as determined by the TGs, ApoB, and TEM analysis.
Thesis - Open Access
Sun, Yuxi, "Development of in vitro Chylomicron Assay Using Caco-2 Cells" (2013). Electronic Theses and Dissertations. Paper 1781. http://dc.etsu.edu/etd/1781
Copyright by the authors.